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Laboratory of Biochemistry PDF Print E-mail

Laboratory of Biochemistry

Dipartimento di Biochimica, Sezione di Medicina, Università di Pavia

via Taramelli 3/B, 27100 Pavia, Italy

 

The research and scientific activity of the Biochemistry Department “A. Castellani” of Pavia University was started at the beginning of 1980. The Department is divided in 3 sections. In particular, the Medicine Section is furnished with the following equipments: tissue culture rooms provided with incubators and laminar flow hoods, autoclaves for sterilization, devices for the production of ultrapure water, biochemistry and molecular biology laboratories (HPLC and FPLC systems, chromatographic columns, balances, pH-meter, rotating platforms, electrophoresis systems for DNA and proteins, trans-illuminator, PCR, Real Time-PCR, Western/Southern Blotting apparatus, refrigerated centrifuges, water baths), optical microscopes equipped with fluorescence and digital camera, fluorimeter, ELISA washer and reader, biosafety cabinets, chemical hoods, a dark room for the development of autoradiography and photography, a radioisotope room; eukaryotic and prokaryotic cells banks kept in nitrogen containers; lyophilizators, microcentrifuges, centrifuges, and ultracentrifuges; -80°C and -20°C freezers; two cold rooms; a library with computers; studies for professors and Ph.D. students.

The research fields are:

  • Analysis of the immunological and biochemical properties of bacterial adhesins for components of the extracellular matrix proteins
  • Interaction of different type of biomaterials with eukaryotic cells such as human osteoblasts, fibroblasts, platelets and macrophages (biocompatibility and citotoxicity studies)
  • Tissue engineering studies using stem cells and different type of scaffolds
  • Studies on bacterial infection (caused by S.aureus, S.epidermidis and E.coli strains) to biomaterials and strategies to avoid such infections