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Laboratory of Development Biology PDF Print E-mail

Laboratory of Development Biology

Dipartimento di Biologia Animale, Università di Pavia

piazza Botta 9, 27100 Pavia, Italy

 

The studies on the developmental competence of gametes and embryos have layed the conceptual bases for the understanding of pluripotency and cell differentiation. This research is relevant for possible biomedical applications in the development of novel cell therapies for the treatment of degenerative diseases. Research at the Laboratory of Development Biology is focused on the comprehesion of the molecular bases of cell pluripotency. Our laboratory has a long-standing interest in the mechanisms regulating the differentiation of gametes. We demonstrated that some of the molecular factors involved in the acquisition of the oocyte’s developmental competence are also critical for the maintenance of cell pluripotency.

The research fields are:

  • Identification of morphological and molecular markers that define the mammalian oocyte’s developmental competence
  • Analysis of the pathways of gene expression in oocytes during folliculogenesis, also following the alteration of the expression of genes involved in the acquisition of the oocyte’s developmental competence
  • Analysis of the meiotic silencing of unsynapsed chromatin during male meiosis in mice carriers of chromosomal structural heterozygosities
  • Study of the molecular mechanisms that alter the differentation of embryonic stem cells into cardiomiocytes cultered in the presence of xenobiotics (e.g., dioxin)
  • Pluripotent stem cells (e.g., embryonic stem cells or induced pluripotent stem cells, iPS) as in vitro models for the study of thrombopoiesis and inherited thrombocytopenias (in collaboration with dr. A. Balduini and prof. C. Balduini, University of Pavia)
  • Biological effets of radiations on pluripotent stem cells

The technical skills are:

  • Histology
  • Immunohistochemistry
  • Immunoblotting
  • DNA and RNA extraction
  • PCR, RT-PCR, real-time PCR
  • Southern blotting
  • Transfection
  • DNA and RNA in situ hybridisation
  • Cytogenetics
  • Cell culture including embryonic stem cells, iPS cells, oocytes and embryos
  • In vitro fertilisation
  • Gamete and preimplantation embryos micromanipulation
  • Nuclear transfer

The core facilities are:

  • The Laboratory has access to the animal house of the University of Pavia equipped to breed and manipulate several strains of mice including immunodeficient mice
  • A sterile surgical room perfectly equipped is also available
  • Room for the use of radioactive material